Protein Expression
نویسندگان
چکیده
We show in vitro synthesis of a fully functional protein that binds substrate with high affinity. After reconstitution into proteoliposomes, the in vitro synthesized protein actively transports substrate. Coexpression of two plasmids in the Rapid Translation System RTS allowed the demonstration of functional complementation. Pull-down experiments confirmed that the basic functional unit of EmrE is the dimer.
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تاریخ انتشار 2004